Document 0525
 DOCN  M9480525
 TI    Quantitative analysis of viral burden in tissues from adults and
       children with symptomatic human immunodeficiency virus type 1 infection
       assessed by polymerase chain reaction.
 DT    9410
 AU    Sei S; Kleiner DE; Kopp JB; Chandra R; Klotman PE; Yarchoan R; Pizzo PA;
       Mitsuya H; Medicine Branch, National Cancer Institute, NIH, Bethesda,
       MD; 20892.
 SO    J Infect Dis. 1994 Aug;170(2):325-33. Unique Identifier : AIDSLINE
       MED/94308597
 AB    The amount of human immunodeficiency virus type 1 (HIV-1) in various
       tissues was investigated by polymerase chain reaction (PCR) in 16
       patients with end-stage HIV-1 infection and 7 patients with symptomatic
       but less advanced disease. During postmortem study of the 16 end-stage
       patients, HIV-1 DNA was found most often in lymph nodes and the spleen
       (both 100%), lung (93.8%), and colon (87.5%). Biopsied lymph nodes from
       the 7 symptomatic patients contained substantially higher copy numbers
       of HIV-1 RNA and DNA than did peripheral blood mononuclear cells (PBMC).
       Plasma viral RNA levels correlated significantly with the amount of
       HIV-1 RNA in PBMC (r2 = .86, P = .0025) but not with the level of viral
       RNA in lymph nodes in patients with symptomatic HIV-1 infection. These
       data suggest that although lymph nodes represent the main site for HIV-1
       infection and replication, the level of circulating viral burden may not
       be solely determined by the magnitude of active HIV-1 replication in
       lymph nodes.
 DE    Acquired Immunodeficiency Syndrome/*MICROBIOLOGY/MORTALITY  Adult
       Central Nervous System/MICROBIOLOGY  Child  Child, Preschool
       Colon/MICROBIOLOGY  DNA, Viral/*ANALYSIS  Female  Gene Expression
       Regulation, Viral  Human  HIV-1/*GROWTH &
       DEVELOPMENT/GENETICS/PHYSIOLOGY  Infant  Leukocytes,
       Mononuclear/MICROBIOLOGY  Lung/MICROBIOLOGY  Lymph Nodes/*MICROBIOLOGY
       Lymphoid Tissue/MICROBIOLOGY  Male  Middle Age  Polymerase Chain
       Reaction  RNA, Messenger/BIOSYNTHESIS  RNA, Viral/ANALYSIS/BLOOD
       Spleen/*MICROBIOLOGY  Thymus Gland/MICROBIOLOGY  Viremia/MICROBIOLOGY
       Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).